The binding reaction is initiated by the addition of a polyclonal antibody to E1S to each well.Īfter a 2 hour incubation the plate is washed and substrate is added. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies.Īn E1S-peroxidase conjugate is added to the standards and samples in the wells. Please read the complete kit insert before performing this assay.Īn E1S standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. The kit will quantitatively measure E1S present in diluted buffer samples and tissue culture media samples. The end user should evaluate recoveries of E1S in other sample matrices being tested. Samples containing visible particulate should be centrifuged prior to using.Įstrone-3-sulfate can be as- sayed in solid sample types by using one of the extraction protocols lit.asp.Įstrone-3-sulfate (E1S) is identical across all species and we expect this kit to measure estrone-3- sulfate from all sources. This assay has been validated for serum, plasma, fecal, urine and tissue culture samples. Software for converting raw relative optical density readings from the plate reader and carrying out four parameter logistic curve (4PLC) fitting.Ĭontact your plate reader manufacturer for de- tails. Repeater pipet with disposable tips capable of dispensing 25 μL, 50 μL and 100 μL.Ĭolorimetric 96 well microplate reader capable of reading optical density at 450 nm. Stop Solution A 1M solution of hydrochloric acid. Wash Buffer Concentrate A 20X concentrate that should be diluted with deionized or distilled water. 28 mL Or 55 mLĭissociation Reagent is to be used only with Serum and Plasma samples. 3 mL Or 13 mL Or -13ML Assay Buffer Concentrate A 5X concentrate that should be diluted with deionized or distilled water. 3 mL Or 13 mL Or -13ML DetectX® Estrone-3-Sulfate (E1S) Conjugate An Estrone-3-Sulfate-peroxidase conjugate in a special stabilizing solution. 125 μL Or 625 μLĭetectX® Estrone-3-Sulfate (E1S) Antibody A rabbit polyclonal antibody specific for Estrone-3-Sulfate. 1 Or 5 EachĮstrone-3-Sulfate (E1S) Standard Estrone-3-Sulfate (E1S) at 40,000 pg/mL in a special stabilizing solution. Steroid Cross Reactivity: Estrone-3-sulfate 100 %, Estrone 267 %, Estrone-3-glucuronide 200 %, 17-Estradiol 11.7 %, Estradiol-3-Glucuronide 5.7 %, Estradiol-3-Sulfate 5.0 %, Estradiol-17-Sulfate 0.2 %, Progesterone < 0.2 %, Estriol < 0.2 %, Cortisol < 0.2 %, Testosterone < 0.2 %, Pregnanediol < 0.2 %Ĭoated Clear 96 Well Plates Clear 1 by 8 break-apart strip well plastic microtiter plate(s) coated with goat anti-rabbit IgG. The following cross reactants were tested in the assay and calculated at the 50 % binding point. The DetectX® Estrone-3-Sulfate (E1S) Immunoassay kit uses a specifically generated antibody to measure E1S in a variety of matrices, including serum, plasma, urine and fecal samples.įecal, Urine, Serum, Plasma, Tissue Culture Medium Custom Recombinant Antibody (rAbs) Services.Annexin V-FITC Apoptosis Detection Kits.
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